Input Gene Sequence and Design Probes

Enter Gene Name: Enter Gene Sequence (minimum 102 bp): Select Amplifier: B1B2B3 B4B5B6 B7B8B9 B10B11B12 B13B14B15 B16B17B18 B19B20 Select GC Content Threshold (%): 30% 35% 40% 45% 50% 55% 60% 65% 70% Enter Gap between Probe Sets (in base pairs): Design Probes Download Excel File

Cite: Banerjee, T. D., Raine, J., Mathuru, A. S., Chen, K. H., and Monteiro, A. (2024). Spatial mRNA profiling using Rapid Amplified Multiplexed-FISH (RAM-FISH). bioRxiv. Link

References: 1) Choi, H. M. T., Schwarzkopf, M., Fornace, M. E., Acharya, A., Artavanis, G., Stegmaier, J., Cunha, A. and Pierce, N. A. (2018). Third-generation in situ hybridization chain reaction : multiplexed , quantitative , sensitive , versatile , robust. Development. Link
2) Wang, Y., Liu, X., Zeng, Y., Saka, S. K., Xie, W., Goldaracena, I., Kohman, R. E., Yin, P. and Church, G. M. (2024). Multiplexed in situ protein imaging using DNA-barcoded antibodies with extended hybridization chain reactions. Nucleic Acids Research 52, e71. Link